Journal: Cellular & molecular biology letters

Article Title: PRDX1 negatively regulates bleomycin-induced pulmonary fibrosis via inhibiting the epithelial-mesenchymal transition and lung fibroblast proliferation in vitro and in vivo.

doi: 10.1186/s11658-023-00460-x

Figure Lengend Snippet: Fig. 7 Expression of EMT and fibrosis-related proteins after BLM treatment in mice. Lung fibrosis was induced in mice by intratracheal administration of BLM (5 mg/kgBW in 50 μL PBS) for 21 days. A Expression of EMT (N-cadherin, E-cadherin, vimentin, slug), collagen-related (α-SMA, fibronectin, collagen I/III), cell cycle (P16, P21), and PCNA proteins in mouse lung tissues as measured by western blotting. B–M Quantitative analyses of the data presented in panel A. *, p < 0.05; **, p < 0.01; ***, p < 0.001. EMT epithelial-mesenchymal transition, BLM bleomycin, PBS phosphate-buffered saline, α-SMA α-smooth muscle actin, PCNA proliferating cell nuclear antigen

Article Snippet: Phosphate-buffered saline (PBS; SH30256.01B) and Dulbecco’s modified Eagle’s medium (DMEM; SH30243.01) were obtained from Cytiva (Marlborough, MA, USA); dihydroethidium (DHE; ID3560), mitochondrial superoxide (Mito-SOX; M36008), JC-1 (M8650), and Hoechst (C0031) reagents from Solarbio Science and Technology Co., Ltd. (Beijing, China); propidium iodide (PI; C1052) from Beyotime Biotechnology Co., Ltd. (Nanjing, China); penicillin/streptomycin (P/S; P7630), trypsin/EDTA solution (TE; T1300), and foetal bovine serum (FBS; #11011-8611) from Solarbio Science and Technology Co., Ltd. (Beijing, China); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; #298-93-1) from Amresco, VWR Life Science (Radnor, PA, USA); dimethyl sulfoxide (DMSO; D1435) from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany); BLM (R25001) from Thermo Fisher Scientific, Inc. (Waltham, MA, USA); the antibodies for β-actin (sc-4778), N-cadherin (sc-8424), E-cadherin (sc-7870), vimentin (c-7870), fibronectin (sc-8422), and slug (sc-166476) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); TGF-β1 enzyme-linked immunosorbent assay kit (ELISA; DB100C) from R&D Systems, Inc. (Minneapolis, MN, USA); and Cell Cycle Analysis Kit (C1052) from Beyotime Biotechnology (Shanghai, China).

Techniques: Expressing, Western Blot, Saline